To evaluate the potential effects Ankaferd Blood Stopper (ABS) and oxytocin (OT) in an experimental model of endometriosis, 18 female Sprague Dawley rats were used in this study. The animals were randomly divided into three groups after surgical induction of endometriosis: group 1: control group (isotonic saline, 1 mL / kg / day, intramuscular, n = 6); Group 2: PL group (OT, 80 U / kg / day, intramuscular, n = 6); group 3 : a group of ABS (ABS, 1.5 mL / kg / day, intraperitoneal, n = 6). Each group was treated for four weeks (twice per week).

Endometriosis explant volume is measured in biopsy samples for histopathological analysis. Vascular endothelial growth factor (VEGF), monocytes chemotactic protein level -1 (MCP-1) and tumor necrosis factor (TNF-α) measured in plasma and peritoneal fluid. endometriosis explants volume significantly decreased after administration of OT (P <0.0001). Epithelial score was significantly decreased in both treatment groups compared with the control group (P <0.05). TUNEL immunohistochemistry showed more changes in endometriosis focus apoptosis (epithelial gland and surrounding tissue) in the PL group than the control group (P <0.05).

The level of VEGF, MCP-1 and TNF-α were significantly reduced in the OT group (P <0.05), whereas no significant change in the protein to levels found in the group of ABS-applied. Results showed that OT has a greater potential as a therapeutic agent in experimentally induced peritoneal endometriosis, in which the ABS, which is a modulator of VEGF, appear to act through different mechanisms to demonstrate palliative effect in a mouse model of peritoneal endometriosis.

The Role of Ankaferd Blood Stopper and Oxytocin as Potential Therapeutic Agents in Endometriosis: A Rat Model
The Role of Ankaferd Blood Stopper and Oxytocin as Potential Therapeutic Agents in Endometriosis: A Rat Model

Sphingosine 1-phosphate / microRNA-1249-5p / MCP-1 axis is involved in the inflammation associated macrophage fatty liver injury in mice

monocytes chemotactic protein -1 (MCP-1) is one of the most representative inflammatory cytokines, and has proven to markedly increased in liver injury and sphingosine 1-phosphate (S1P) -treated macrophages. However, microRNAs (miRNAs) are targeting MCP-1 and the role of miRNA / MCP-1 axis in S1P-mediated liver inflammation remains unknown. Here we show that the expression of MCP-1 increased in the liver and macrophages isolated rat heart MCDHF. In addition, there is a positive correlation between the degree of hepatic S1P and MCP-1.

We then predict the MCP-1 targeting miRNAs with bioinformatics analyzes and miRNA-1249-5p select (mir-1249-5p) from the intersection of database TargetScan and down-regulated miRNAs in the liver injured. S1P significantly up-regulate the expression of MCP-1 and miR-1249-5p decrease in expression in macrophages. MiR-1249-5p directly target of 3 ‘- UTR of MCP-1 and negatively regulates the expression of the S1P-treated macrophages. Mir-1249-5p agomir administration decreased liver MCP-1 levels and attenuates hepatic inflammation in mice MCDHF.

Protein – protein interaction network by displaying STRING that S1P system is closely related to MCP-1 / CCR2 axis in inflammatory tissue. In conclusion, we characterize the important role of miR-1249-5p in negatively regulates the expression of MCP-1 in vitro and in vivo, which can open up new perspectives for pharmacological treatment of heart disease. This article is protected by copyright. All rights reserved.Single nucleotide polymorphisms in miRNA binding sites (mir-SNP) that are associated with cancer risk.

(RS)-MCPG

B6265-100 100 mg
EUR 1497

(RS)-MCPG

B6265-25 25 mg
EUR 502

(RS)-MCPG

B6265-5 5 mg
EUR 195

(RS)-MCPG

B6265-5.1 10 mM (in 1mL DMSO)
EUR 209

(RS)-MCPG

B6265-50 50 mg
EUR 846

(S)-MCPG

B6266-10 10 mg
EUR 176

(S)-MCPG

B6266-25 25 mg
EUR 350

(S)-MCPG

B6266-5 5 mg
EUR 116

(RS)-MCPG

HY-100371 100mg
EUR 946

(S)-MCPG

HY-100406 5mg
EUR 133

(RS)-MCPG disodium salt

B7476-10 10 mg
EUR 302

(RS)-MCPG disodium salt

B7476-50 50 mg
EUR 1114

We assessed the association between five miR-SNP in the 3 ‘translated regions ( 3 ‘ – UTR) of RYR 3 (rs1044129), KIAA042 3 (rs105 3 667), C14orf101 (rs4901706), GOLGA7 < / i> (rs11 3 3 7), and KRT81 (rs 3 660) and breast cancer risk ( BC).