Monocyte chemotactic protein-1 and nitrotyrosine in irritable bowel syndrome
Irritable bowel syndrome (IBS) is one of the most frequent and common functional gastrointestinal disorder that has aetiopathogenesis multifactorial. Several biomarkers have been tested in the search for a reliable and specific biomarkers, but there is no specific biomarkers for IBS. The purpose of this study was to evaluate two different biomarkers putative pathways of pathogenesis of IBS: monocyte chemotactic protein-1 (MCP-1) and nitrotyrosine, in order to establish their role as potential biomarkers.
We enrolled 42 consecutive patients diagnosed with IBS by Rome III criteria and 35 healthy controls, respectively. serum concentration of two biomarkers (MCP-1 and nitrotyrosine) is determined by using a commercial ELISA kit. serum levels of MCP-1 were not statistically significantly higher in IBS patients than the control group (204 ± 130 vs 174 ± 73 pg / ml; P = 0.311). nitrotyrosine levels were statistically significantly lowered in IBS patients than the control group (30 ± 12 vs 353 ± 14 nM; P = 0.050).
MCP-1 levels were higher in patients with the metabolic syndrome compared IBS IBS patients without metabolic syndrome (239 ± 153 vs 168 ± 120 pg / ml; P = 0.948) and in control with the metabolic syndrome (174 ± 56 pg / ml). MCP-1 serum levels were statistically significantly higher in IBS patients with the metabolic syndrome compared with controls (239 ± 153 vs 157 ± 89 pg / ml; P = 0.037), showing several factors involved, especially diet and its association with metabolic syndrome, and it shows that MCP-1 coul
Monocyte chemotactic protein-1 and nitrotyrosine in irritable bowel syndrome
Evaluation of Immunomodulatory Ability of Lactic Acid Bacteria Isolated from Cattle Feedlot Against Mastitis Using Bovine Mammary Epithelial Cells In Vitro Assay
bovine mastitis, an inflammation of the mammary gland, affecting the quality and quantity of milk production. mastitis control relies on a combination of one or more antibiotic therapy. Because of the increasing antibiotic resistance in pathogens, intramammary infusion of lactic acid bacteria (LAB) has been considered as a potential alternative to antibiotics to treat and prevent bovine mastitis through the host immune enhancement. the effect of probiotic strain-dependent characteristics;
Therefore, strains of LAB candidate must be evaluated efficiently identify those with the best potential. Here, we examine the LAB strains were originally isolated from the environment fattening cattle on their ability to induce the Toll-like receptor (TLR) -triggered inflammatory response in bovine mammary epithelial (BME) cells in vitro.
BME cells were pre-stimulated with LAB strains individually for 12, 24, and 48 hours and then challenged with Escherichia coli -derived lipopolysaccharide (LPS) for 12 hours. MRNA expression of immune selected gene-interleukin 1 alpha ( IL-1α ), IL-1β , monocytes chemotactic protein 1 ( MCP-1 ), IL-8 , chemokine (CXC motif) ligand 2 ( CXCL2 ), and CXCL 3 as measured by real-time quantitative PCR (RT-qPCR). The results showed that pretreatment with few Lactobacillus strains capable of differentially regulate inflammatory responses in cell LPS BME;
However, the strain-dependent differences are found. The most remarkable effect was found for Lactobacillus acidophilus CRL2074, which reduces the expression of IL-1α , IL-1β , MCP- 1 , IL-8 and CXCL 3 , and Lactobacillus rhamnosus CRL2084 reduced IL-1β , MCP-1 and IL-8 expression.
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