Neuroinflammation has been involved in neurodegenerative diseases and acute brain injury such as stroke. monocytes chemotactic protein -1-induced protein -1 (Mcpip1) is a multifunctional protein action is known to have pro-apoptotic or anti-apoptotic depending on the nature of the experimental arrangement. However, its role in brain damage after asphyxia in the developing brain has not been studied. Therefore, we explored the role Mcpip1 on brain injury after hypoxia-ischemia in neonatal rats.
At postnatal day 7, Mcpip1-deficient and wild-type mice carotid artery ligation and exposure to hypoxia (8% oxygen). After a hypoxic-ischemic insult, we determine the time-course of cell death of apoptosis and expression levels of genes that encode proinflammatory factors. Mcpip1 impact on long-term brain damage rated one week after hypoxia-ischemia by cresyl violet staining.
We found caspase- 3 significantly increased activity in the ipsilateral brain tissue within 12-24 hours after hypoxia-ischemia. There is a marked increase in levels of mRNA transcripts that encode Mcpip1, TNF, and CCL2 in the ipsilateral brain tissue 6-48 hours after hypoxia-ischemia. We found caspase- hypoxia-ischemia-induced 3 and the activity levels of proinflammatory genes are inactivated in Mcpip1-KO mice compared to wild-type mice.
Histological assessment revealed that hypoxia-ischemia-induced brain tissue loss was significantly attenuated in the hippocampus of Mcpip1-KO mice compared to wild-type mice (9.0 ± 5.6% vs 3 3 0.9 ± 11.0%, P <0.05). Our data show that Mcpip1 contributes to acute and delayed brain damage, in part, through the regulation of neuroinflammation after hypoxic-ischemic insult in the developing rat brain.

MCP-1 priming Enhanced Mesenchymal stromal cells Therapeutic Effect on Contact Hypersensitivity by Enabling COX2-PGE2 / STAT3 Pathway mesenchymal stromal
cells (MSC) has become a promising treatment for inflammation-related diseases, and the efficacy of their therapy mainly depends on the crosstalk between the MSC and inflammation. However, methods to improve the efficiency of immunosuppressive of MSC in different diseases still need to be developed. In this study, we investigated whether preconditioning MSC inflammatory cytokines associated with the disease can improve their immunosuppressive properties and increasing the success of therapy.
In a mouse model of contact hypersensitivity (CHS), an inflammatory profile screening revealed that among all the cytokines tested, monocytes chemotactic protein -1 (MCP- 1) exhibited the lowest level increased significantly in the local microenvironment. As expected, MSC asphalt with MCP-1 (P-MSCs) exhibited an enhanced ability to downregulate the secretion of proinflammatory cytokines, induce regulatory T cells, inhibiting T cell proliferation, and the polarization of M2-type macrophages.
In vivo experiments showed that P-MSCs alleviated ear swelling and local production of proinflammatory cytokines is more effective than MSC controls. Mechanically, MCP-1 can significantly activate signal transducer and activator of transcription 3 (STAT 3 ) signaling pathway and induces the expression of cyclooxygenase-2 (COX2) and prostaglandin E2 (PGE2) in the MSC. STAT 3 inhibitor reversed MCP-1-mediated increase their immunosuppressive capabilities.
(RS)-MCPG disodium salt |
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B7476-100 | ApexBio | 100mg | EUR 1125 |
Description: group I/group II metabotropic glutamate receptor antagonist |
(RS)-MCPG disodium salt |
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B7476-25 | ApexBio | 25mg | EUR 348 |
Description: group I/group II metabotropic glutamate receptor antagonist |
(RS)-MCPG disodium salt |
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B7476-5 | ApexBio | 5mg | EUR 114 |
Description: group I/group II metabotropic glutamate receptor antagonist |
ATP disodium salt |
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B3304-5.1 | ApexBio | 10 mM (in 1mL H2O) | EUR 129.6 |
Description: ATP Disodium salt is a P2 purinoceptor agonist. |
ATP disodium salt |
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B3304-50 | ApexBio | 50 mg | EUR 153.6 |
Description: ATP Disodium salt is a P2 purinoceptor agonist. |
ATP disodium salt |
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B3304-S | ApexBio | Evaluation Sample | EUR 97.2 |
Description: ATP Disodium salt is a P2 purinoceptor agonist. |
BCA disodium salt |
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2810-100G | Biovision | each | EUR 1038 |
BCA disodium salt |
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2810-1G | Biovision | each | EUR 144 |
BCA disodium salt |
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2810-500G | Biovision | each | EUR 3126 |
BCA disodium salt |
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2810-5G | Biovision | each | EUR 261.6 |
UDP disodium salt |
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B7278-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 129.6 |
UDP disodium salt |
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B7278-50 | ApexBio | 50 mg | EUR 150 |
ADA disodium salt |
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GB0256-100G | Glentham Life Sciences | 100 g | EUR 132 |
ADA disodium salt |
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GB0256-500G | Glentham Life Sciences | 500 g | EUR 409.2 |
ATP (disodium salt) |
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HY-B0345A | MedChemExpress | 10mM/1mL | EUR 135.6 |
ATP disodium salt |
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B3304-1000 | ApexBio | 1g | EUR 43 |
Description: P2 purinoceptor agonist |
ATP disodium salt |
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B3304-5000 | ApexBio | 5g | EUR 86 |
Description: P2 purinoceptor agonist |
AMP Disodium Salt |
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40190012-1 | Bio-WORLD | 25 g | EUR 75.46 |
AMP Disodium Salt |
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40190012-2 | Bio-WORLD | 100 g | EUR 233.11 |
CTP Disodium Salt |
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40390000-1 | Bio-WORLD | 50 mg | EUR 34.58 |
CTP Disodium Salt |
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40390000-2 | Bio-WORLD | 100 mg | EUR 57.14 |
GTP Disodium salt |
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40790012-1 | Bio-WORLD | 50 mg | EUR 37.26 |
GTP Disodium salt |
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40790012-2 | Bio-WORLD | 100 mg | EUR 71.74 |
ADA disodium salt |
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GB0256-100 | Glentham Life Sciences | 100 | EUR 79.1 |
ADA disodium salt |
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GB0256-500 | Glentham Life Sciences | 500 | EUR 308.5 |
DNQX disodium salt |
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B5288-100 | ApexBio | 100 mg | EUR 334.8 |
DNQX disodium salt |
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B5288-25 | ApexBio | 25 mg | EUR 153.6 |
DNQX disodium salt |
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B5288-50 | ApexBio | 50 mg | EUR 223.2 |
NADH, disodium salt |
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2735-1000 | Biovision | each | EUR 202.8 |
NADH, disodium salt |
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2735-500 | Biovision | each | EUR 170.4 |
NADH, disodium salt |
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2735-5000 | Biovision | each | EUR 652.8 |
NADP, disodium salt |
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2736-100 | Biovision | each | EUR 183.6 |
NADP, disodium salt |
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2736-1000 | Biovision | each | EUR 940.8 |
NADP, disodium salt |
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2736-500 | Biovision | each | EUR 548.4 |
NBQX disodium salt |
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2759-25 | Biovision | each | EUR 770.4 |
NBQX disodium salt |
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2759-5 | Biovision | each | EUR 248.4 |
BCIP disodium salt |
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BB1171 | Bio Basic | 500mg | EUR 279.24 |
NBQX disodium salt |
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B6566-10 | ApexBio | 10 mg | EUR 266.4 |
NBQX disodium salt |
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B6566-25 | ApexBio | 25 mg | EUR 466.8 |
NBQX disodium salt |
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B6566-5 | ApexBio | 5 mg | EUR 166.8 |
NBQX disodium salt |
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B6566-50 | ApexBio | 50 mg | EUR 819.6 |
CNQX disodium salt |
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B6567-1 | ApexBio | 1 mg | EUR 160.8 |
CNQX disodium salt |
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B6567-10 | ApexBio | 10 mg | EUR 258 |
CNQX disodium salt |
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B6567-50 | ApexBio | 50 mg | EUR 895.2 |
NADH (disodium salt) |
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HY-F0001 | MedChemExpress | 10mM/1mL | EUR 135.6 |
NADP (disodium salt) |
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HY-F0002A | MedChemExpress | 500mg | EUR 325.2 |
R788 disodium salt |
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GW0002-1 | Glentham Life Sciences | 1 | EUR 212.7 |
R788 disodium salt |
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GW0002-10 | Glentham Life Sciences | 10 | EUR 533.3 |
R788 disodium salt |
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GW0002-5 | Glentham Life Sciences | 5 | EUR 311.9 |
DNQX disodium salt |
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B5288-10 | ApexBio | 10mg | EUR 113 |
Description: Selective non-NMDA receptor antagonist |
NBQX disodium salt |
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B6566-1 | ApexBio | 1mg | EUR 182 |
Description: A potent, highly selective and competitive AMPA receptor antagonist |
dCTP Disodium Salt |
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40390013-1 | Bio-WORLD | 50 mg | EUR 71.74 |
dCTP Disodium Salt |
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40390013-2 | Bio-WORLD | 100 mg | EUR 139.46 |
NADH Disodium Salt |
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41490008-1 | Bio-WORLD | 1 g | EUR 41.18 |
NADH Disodium Salt |
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41490008-2 | Bio-WORLD | 5 g | EUR 160.14 |
NADH Disodium Salt |
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41490008-3 | Bio-WORLD | 50 g | EUR 1371.71 |
NADH, Disodium Salt |
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A3402-1G | Biomatik Corporation | 1G | EUR 46.2 |
Description: High Purity |
NADH, Disodium Salt |
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A3402-500MG | Biomatik Corporation | 500MG | EUR 28.6 |
Description: High Purity |
NADH, Disodium Salt |
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A3402-5G | Biomatik Corporation | 5G | EUR 184.8 |
Description: High Purity |
NADP, Disodium Salt |
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A4213-100MG | Biomatik Corporation | 100MG | EUR 35.2 |
Description: High Purity |
NADP, Disodium Salt |
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A4213-1G | Biomatik Corporation | 1G | EUR 138.6 |
Description: High Purity |
NADP, Disodium Salt |
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A4213-250MG | Biomatik Corporation | 250MG | EUR 46.2 |
Description: High Purity |
cGAMP disodium salt |
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2435-100 | Biovision | each | EUR 235.2 |
POPSO, disodium salt |
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PB4959 | Bio Basic | 25g | EUR 92.36 |
cGAMP disodium salt |
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GL2101-100UG | Glentham Life Sciences | 100 ug | EUR 192 |
cGAMP disodium salt |
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GL2101-500UG | Glentham Life Sciences | 500 ug | EUR 398.4 |
PIPES disodium salt |
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GB1799-100G | Glentham Life Sciences | 100 g | EUR 132 |
PIPES disodium salt |
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GB1799-250G | Glentham Life Sciences | 250 g | EUR 217.2 |
PIPES disodium salt |
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GB1799-25G | Glentham Life Sciences | 25 g | EUR 84 |
PIPES disodium salt |
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GB1799-500G | Glentham Life Sciences | 500 g | EUR 370.8 |
POPSO disodium salt |
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GB6170-100G | Glentham Life Sciences | 100 g | EUR 322.8 |
POPSO disodium salt |
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GB6170-25G | Glentham Life Sciences | 25 g | EUR 151.2 |
PIPES disodium salt |
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GB1799-100 | Glentham Life Sciences | 100 | EUR 79.1 |
PIPES disodium salt |
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GB1799-25 | Glentham Life Sciences | 25 | EUR 33.1 |
PIPES disodium salt |
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GB1799-250 | Glentham Life Sciences | 250 | EUR 150.4 |
PIPES disodium salt |
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GB1799-500 | Glentham Life Sciences | 500 | EUR 276.9 |
POPSO disodium salt |
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GB6170-100 | Glentham Life Sciences | 100 | EUR 237.2 |
POPSO disodium salt |
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GB6170-25 | Glentham Life Sciences | 25 | EUR 95 |
cGAMP disodium salt |
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GL2101-100 | Glentham Life Sciences | 100 | EUR 139.6 |
cGAMP disodium salt |
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GL2101-500 | Glentham Life Sciences | 500 | EUR 340.2 |
Cefotetan disodium salt |
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GA5476-100MG | Glentham Life Sciences | 100 mg | EUR 103.2 |
Cefotetan disodium salt |
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GA5476-1G | Glentham Life Sciences | 1 g | EUR 217.2 |
Cefotetan disodium salt |
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GA5476-1 | Glentham Life Sciences | 1 | EUR 150.4 |
Cefotetan disodium salt |
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GA5476-100 | Glentham Life Sciences | 100 | EUR 55.4 |
Etidronate disodium salt |
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GP3560-1G | Glentham Life Sciences | 1 g | EUR 208.8 |
Lobenzarit disodium salt |
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GP3753-100MG | Glentham Life Sciences | 100 mg | EUR 274.8 |
Methoxatin (disodium salt) |
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HY-100196A | MedChemExpress | 10mM/1mL | EUR 223.2 |
Etidronate disodium salt |
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GP3560-1 | Glentham Life Sciences | 1 | EUR 142.4 |
Lobenzarit disodium salt |
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GP3753-100 | Glentham Life Sciences | 100 | EUR 197.7 |
CL 316243 disodium salt |
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B6766-1 | ApexBio | 1 mg | EUR 130.8 |
Description: EC50: (3.0±0.3) X 10-8 M for ?3 adrenoceptor [1].Benzodioxole-containing phenethanolamine CL-316,243 is a murine-selective ?3 adrenoceptor agonist which can correct obesity and elevated blood glucose in diabetic rodents. |
CL 316243 disodium salt |
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B6766-10 | ApexBio | 10 mg | EUR 351.6 |
Description: EC50: (3.0±0.3) X 10-8 M for ?3 adrenoceptor [1].Benzodioxole-containing phenethanolamine CL-316,243 is a murine-selective ?3 adrenoceptor agonist which can correct obesity and elevated blood glucose in diabetic rodents. |
CL 316243 disodium salt |
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B6766-5 | ApexBio | 5 mg | EUR 247.2 |
Description: EC50: (3.0±0.3) X 10-8 M for ?3 adrenoceptor [1].Benzodioxole-containing phenethanolamine CL-316,243 is a murine-selective ?3 adrenoceptor agonist which can correct obesity and elevated blood glucose in diabetic rodents. |
Fluorescein, disodium salt |
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FB0203 | Bio Basic | 100g | EUR 76.7 |
Ticarcillin disodium salt |
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GA1874-100MG | Glentham Life Sciences | 100 mg | EUR 60 |
Ticarcillin disodium salt |
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GA1874-1G | Glentham Life Sciences | 1 g | EUR 132 |
Ticarcillin disodium salt |
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GA1874-500MG | Glentham Life Sciences | 500 mg | EUR 93.6 |
Balsalazide disodium salt |
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GP7647-1G | Glentham Life Sciences | 1 g | EUR 132 |
Fluorescein Disodium Salt |
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40600048-1 | Bio-WORLD | 500 g | EUR 154.1 |
Fluorescein Disodium Salt |
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40600048-2 | Bio-WORLD | 1 kg | EUR 272.69 |
Ticarcillin disodium salt |
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42010008-1 | Bio-WORLD | 1 g | EUR 51.77 |
Ticarcillin disodium salt |
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42010008-2 | Bio-WORLD | 5 g | EUR 89.68 |
Ticarcillin disodium salt |
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42010008-3 | Bio-WORLD | 10 g | EUR 146.33 |
Ticarcillin disodium salt |
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42010008-4 | Bio-WORLD | 25 g | EUR 329.23 |
Balsalazide disodium salt |
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GP7647-1 | Glentham Life Sciences | 1 | EUR 79.1 |
Ticarcillin disodium salt |
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GA1874-1 | Glentham Life Sciences | 1 | EUR 79.1 |
Ticarcillin disodium salt |
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GA1874-100 | Glentham Life Sciences | 100 | EUR 19.8 |
Ticarcillin disodium salt |
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GA1874-500 | Glentham Life Sciences | 500 | EUR 47.4 |
Hypoxanthine Disodium Salt |
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B1185-1G | Biovision | each | EUR 130.8 |
Hypoxanthine Disodium Salt |
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B1185-5G | Biovision | each | EUR 288 |
Phosphomycin disodium salt |
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GA7173-1G | Glentham Life Sciences | 1 g | EUR 84 |
Phosphomycin disodium salt |
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GA7173-25G | Glentham Life Sciences | 25 g | EUR 346.8 |
Phosphomycin disodium salt |
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GA7173-5G | Glentham Life Sciences | 5 g | EUR 160.8 |
Phosphomycin disodium salt |
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GA7173-1 | Glentham Life Sciences | 1 | EUR 39.4 |
Phosphomycin disodium salt |
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GA7173-25 | Glentham Life Sciences | 25 | EUR 257 |
Phosphomycin disodium salt |
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GA7173-5 | Glentham Life Sciences | 5 | EUR 102.7 |
Carbenicillin, Disodium Salt |
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A2511-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 129.6 |
Description: Carbenicillin inhibits the cell-wall synthesis (peptidoglycan cross-linking) by inactivating transpeptidase on the inner surface of the bacterial cell membrane.Carbenicillin is a white to slightly yellow, hygroscopic powder soluble in water and in alcohol. |
Carbenicillin, Disodium Salt |
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A2511-50 | ApexBio | 50 mg | EUR 157.2 |
Description: Carbenicillin inhibits the cell-wall synthesis (peptidoglycan cross-linking) by inactivating transpeptidase on the inner surface of the bacterial cell membrane.Carbenicillin is a white to slightly yellow, hygroscopic powder soluble in water and in alcohol. |
Carbenicillin disodium salt |
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GA1299-10G | Glentham Life Sciences | 10 g | EUR 274.8 |
Carbenicillin disodium salt |
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GA1299-1G | Glentham Life Sciences | 1 g | EUR 103.2 |
Carbenicillin disodium salt |
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GA1299-250MG | Glentham Life Sciences | 250 mg | EUR 70.8 |
Carbenicillin disodium salt |
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GA1299-25G | Glentham Life Sciences | 25 g | EUR 409.2 |
Carbenicillin disodium salt |
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GA1299-5G | Glentham Life Sciences | 5 g | EUR 189.6 |
Carbenicillin, Disodium Salt |
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A2511-1000 | ApexBio | 1g | EUR 67 |
Description: Inhibits the cell-wall synthesis,antibiotic |
Carbenicillin, Disodium Salt |
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A2511-5000 | ApexBio | 5g | EUR 243 |
Description: Inhibits the cell-wall synthesis,antibiotic |
Carbenicillin Disodium Salt |
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40310000-1 | Bio-WORLD | 1 g | EUR 25.61 |
Carbenicillin Disodium Salt |
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40310000-2 | Bio-WORLD | 5 g | EUR 51.79 |
Carbenicillin Disodium Salt |
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40310000-3 | Bio-WORLD | 10 g | EUR 81.44 |
Carbenicillin Disodium Salt |
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40310000-4 | Bio-WORLD | 25 g | EUR 142.47 |
Carbenicillin Disodium Salt |
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40310000-5 | Bio-WORLD | 100 g | EUR 467.78 |
Carbenicillin, Disodium Salt |
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A2311-1G | Biomatik Corporation | 1G | EUR 71.5 |
Description: Ultra Pure |
Carbenicillin, Disodium Salt |
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A2311-5G | Biomatik Corporation | 5G | EUR 255.2 |
Description: Ultra Pure |
Carbenicillin disodium salt |
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GA1299-1 | Glentham Life Sciences | 1 | EUR 55.4 |
Carbenicillin disodium salt |
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GA1299-10 | Glentham Life Sciences | 10 | EUR 197.7 |
Carbenicillin disodium salt |
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GA1299-25 | Glentham Life Sciences | 25 | EUR 308.5 |
Carbenicillin disodium salt |
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GA1299-250 | Glentham Life Sciences | 250 | EUR 28.4 |
Carbenicillin disodium salt |
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GA1299-5 | Glentham Life Sciences | 5 | EUR 126.5 |
Phosphoramidon Disodium Salt |
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B4790-25 | ApexBio | 25 mg | EUR 588 |
Description: Phosphoramidon Disodium Salt is a potent inhibitor of metalloproteinase [1]. Metalloproteinase is an enzyme whose catalytic mechanism involves a metal. Most metalloproteases require zinc and some require cobalt. |
Phosphoramidon Disodium Salt |
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B4790-5 | ApexBio | 5 mg | EUR 226.8 |
Description: Phosphoramidon Disodium Salt is a potent inhibitor of metalloproteinase [1]. Metalloproteinase is an enzyme whose catalytic mechanism involves a metal. Most metalloproteases require zinc and some require cobalt. |
Protoporphyrin. disodium salt |
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P160-100MG | TOKU-E | 100 mg | EUR 140.46 |
Description: C34H34N4O4.Na2 |
Protoporphyrin. disodium salt |
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P160-25MG | TOKU-E | 25 mg | EUR 40.32 |
Description: C34H34N4O4.Na2 |
Collectively, our findings indicate that MCP-1 related CHS conditioning increases immunomodulatory effects of MSC and enhanced their therapeutic efficacy in CHS. Improving the effectiveness of immunosuppressive MSC by preconditioning inflammatory cytokines associated with a particular disease may provide new strategies for MSC-based therapy for inflammatory disease.