Neuroinflammation has been involved in neurodegenerative diseases and acute brain injury such as stroke. monocytes chemotactic protein -1-induced protein -1 (Mcpip1) is a multifunctional protein action is known to have pro-apoptotic or anti-apoptotic depending on the nature of the experimental arrangement. However, its role in brain damage after asphyxia in the developing brain has not been studied. Therefore, we explored the role Mcpip1 on brain injury after hypoxia-ischemia in neonatal rats.

At postnatal day 7, Mcpip1-deficient and wild-type mice carotid artery ligation and exposure to hypoxia (8% oxygen). After a hypoxic-ischemic insult, we determine the time-course of cell death of apoptosis and expression levels of genes that encode proinflammatory factors. Mcpip1 impact on long-term brain damage rated one week after hypoxia-ischemia by cresyl violet staining.

We found caspase- 3 significantly increased activity in the ipsilateral brain tissue within 12-24 hours after hypoxia-ischemia. There is a marked increase in levels of mRNA transcripts that encode Mcpip1, TNF, and CCL2 in the ipsilateral brain tissue 6-48 hours after hypoxia-ischemia. We found caspase- hypoxia-ischemia-induced 3 and the activity levels of proinflammatory genes are inactivated in Mcpip1-KO mice compared to wild-type mice.

Histological assessment revealed that hypoxia-ischemia-induced brain tissue loss was significantly attenuated in the hippocampus of Mcpip1-KO mice compared to wild-type mice (9.0 ± 5.6% vs 3 3 0.9 ± 11.0%, P <0.05). Our data show that Mcpip1 contributes to acute and delayed brain damage, in part, through the regulation of neuroinflammation after hypoxic-ischemic insult in the developing rat brain.

Monocyte chemotactic protein-1-induced protein 1 contributes to neuronal injury following hypoxic-ischemia in the neonatal mouse brain
Monocyte chemotactic protein-1-induced protein 1 contributes to neuronal injury following hypoxic-ischemia in the neonatal mouse brain

MCP-1 priming Enhanced Mesenchymal stromal cells Therapeutic Effect on Contact Hypersensitivity by Enabling COX2-PGE2 / STAT3 Pathway mesenchymal stromal

cells (MSC) has become a promising treatment for inflammation-related diseases, and the efficacy of their therapy mainly depends on the crosstalk between the MSC and inflammation. However, methods to improve the efficiency of immunosuppressive of MSC in different diseases still need to be developed. In this study, we investigated whether preconditioning MSC inflammatory cytokines associated with the disease can improve their immunosuppressive properties and increasing the success of therapy.

In a mouse model of contact hypersensitivity (CHS), an inflammatory profile screening revealed that among all the cytokines tested, monocytes chemotactic protein -1 (MCP- 1) exhibited the lowest level increased significantly in the local microenvironment. As expected, MSC asphalt with MCP-1 (P-MSCs) exhibited an enhanced ability to downregulate the secretion of proinflammatory cytokines, induce regulatory T cells, inhibiting T cell proliferation, and the polarization of M2-type macrophages.

In vivo experiments showed that P-MSCs alleviated ear swelling and local production of proinflammatory cytokines is more effective than MSC controls. Mechanically, MCP-1 can significantly activate signal transducer and activator of transcription 3 (STAT 3 ) signaling pathway and induces the expression of cyclooxygenase-2 (COX2) and prostaglandin E2 (PGE2) in the MSC. STAT 3 inhibitor reversed MCP-1-mediated increase their immunosuppressive capabilities.

(RS)-MCPG disodium salt

B7476-25 25mg
EUR 278
Description: group I/group II metabotropic glutamate receptor antagonist

(RS)-MCPG disodium salt

B7476-5 5mg
EUR 91
Description: group I/group II metabotropic glutamate receptor antagonist

(RS)-MCPG disodium salt

B7476-50 50 mg
EUR 490
Description: group I/group II metabotropic glutamate receptor antagonist

(RS)-MCPG disodium salt

T23270-10mg 10mg Ask for price
Description: (RS)-MCPG disodium salt

(RS)-MCPG disodium salt

T23270-1g 1g Ask for price
Description: (RS)-MCPG disodium salt

(RS)-MCPG disodium salt

T23270-1mg 1mg Ask for price
Description: (RS)-MCPG disodium salt

(RS)-MCPG disodium salt

T23270-50mg 50mg Ask for price
Description: (RS)-MCPG disodium salt

(RS)-MCPG disodium salt

T23270-5mg 5mg Ask for price
Description: (RS)-MCPG disodium salt

BCA disodium salt

2810-100G each
EUR 1038

BCA disodium salt

2810-1G each
EUR 144

BCA disodium salt

2810-500G each
EUR 3126

BCA disodium salt

2810-5G each
EUR 261.6

CTP Disodium Salt

40390000-1 50 mg
EUR 34.58

CTP Disodium Salt

40390000-2 100 mg
EUR 57.14

AMP Disodium Salt

40190012-1 25 g
EUR 75.46

AMP Disodium Salt

40190012-2 100 g
EUR 233.11

GTP Disodium salt

40790012-1 50 mg
EUR 37.26

GTP Disodium salt

40790012-2 100 mg
EUR 71.74

ADA disodium salt

GB0256-100 100
EUR 79.1

ADA disodium salt

GB0256-100G 100 g
EUR 132

ADA disodium salt

GB0256-500 500
EUR 308.5

ADA disodium salt

GB0256-500G 500 g
EUR 409.2

UDP disodium salt

B7278-5.1 10 mM (in 1mL DMSO)
EUR 40
Description: Endogenous P2Y receptor agonist

UDP disodium salt

B7278-50 50 mg
EUR 28
Description: Endogenous P2Y receptor agonist

ATP disodium salt

B3304-1000 1g
EUR 34
Description: P2 purinoceptor agonist

ATP disodium salt

B3304-5.1 10 mM (in 1mL H2O)
EUR 40
Description: P2 purinoceptor agonist

ATP disodium salt

B3304-50 50 mg
EUR 153.6
Description: ATP Disodium salt is a P2 purinoceptor agonist.

ATP disodium salt

B3304-5000 5g
EUR 69
Description: P2 purinoceptor agonist

ATP disodium salt

B3304-S Evaluation Sample
EUR 22
Description: P2 purinoceptor agonist

ATP (disodium salt)

HY-B0345A 10mM/1mL
EUR 135.6

ATP disodium salt

T1352-10mg 10mg Ask for price
Description: ATP disodium salt

ATP disodium salt

T1352-1g 1g Ask for price
Description: ATP disodium salt

ATP disodium salt

T1352-1mg 1mg Ask for price
Description: ATP disodium salt

ATP disodium salt

T1352-50mg 50mg Ask for price
Description: ATP disodium salt

ATP disodium salt

T1352-5mg 5mg Ask for price
Description: ATP disodium salt

NADP disodium salt

111539 10.0mg Ask for price

NADH, disodium salt

2735-1000 each
EUR 202.8

NADH, disodium salt

2735-500 each
EUR 170.4

NADH, disodium salt

2735-5000 each
EUR 652.8

NADP, disodium salt

2736-100 each
EUR 183.6

NADP, disodium salt

2736-1000 each
EUR 940.8

NADP, disodium salt

2736-500 each
EUR 548.4

NBQX disodium salt

2759-25 each
EUR 770.4

NBQX disodium salt

2759-5 each
EUR 248.4

dCTP Disodium Salt

40390013-1 50 mg
EUR 71.74

dCTP Disodium Salt

40390013-2 100 mg
EUR 139.46

NADH Disodium Salt

41490008-1 1 g
EUR 41.18

NADH Disodium Salt

41490008-2 5 g
EUR 160.14

NADH Disodium Salt

41490008-3 50 g
EUR 1371.71

EDTA, DISODIUM SALT

E410 100G
EUR 78.89

NADH, Disodium Salt

A3402-1G 1G
EUR 46.2
Description: High Purity

NADH, Disodium Salt

A3402-500MG 500MG
EUR 28.6
Description: High Purity

NADH, Disodium Salt

A3402-5G 5G
EUR 184.8
Description: High Purity

NADP, Disodium Salt

A4213-100MG 100MG
EUR 35.2
Description: High Purity

NADP, Disodium Salt

A4213-1G 1G
EUR 138.6
Description: High Purity

NADP, Disodium Salt

A4213-250MG 250MG
EUR 46.2
Description: High Purity

DNQX disodium salt

B5288-10 10mg
EUR 90
Description: Selective non-NMDA receptor antagonist

DNQX disodium salt

B5288-100 100 mg
EUR 334.8

DNQX disodium salt

B5288-25 25 mg
EUR 153.6

DNQX disodium salt

B5288-50 50 mg
EUR 396
Description: Selective non-NMDA receptor antagonist

BCIP disodium salt

BB1171 500mg
EUR 279.24

NBQX disodium salt

B6566-1 1mg
EUR 146
Description: A potent, highly selective and competitive AMPA receptor antagonist

NBQX disodium salt

B6566-10 10 mg
EUR 302
Description: A potent, highly selective and competitive AMPA receptor antagonist

NBQX disodium salt

B6566-25 25 mg
EUR 466.8

NBQX disodium salt

B6566-5 5 mg
EUR 166.8

NBQX disodium salt

B6566-50 50 mg
EUR 1271
Description: A potent, highly selective and competitive AMPA receptor antagonist

CNQX disodium salt

B6567-1 1 mg
EUR 160.8

CNQX disodium salt

B6567-10 10 mg
EUR 59
Description: AMPA/kainate antagonist

CNQX disodium salt

B6567-50 50 mg
EUR 167
Description: AMPA/kainate antagonist

CNQX Disodium Salt

C600003 100mg
EUR 1800
Description: 479347-85-8

R788 disodium salt

GW0002-1 1
EUR 212.7

R788 disodium salt

GW0002-10 10
EUR 533.3

R788 disodium salt

GW0002-5 5
EUR 311.9

NADP disodium salt

T22446-10mg 10mg Ask for price
Description: NADP disodium salt

NADP disodium salt

T22446-1g 1g Ask for price
Description: NADP disodium salt

NADP disodium salt

T22446-1mg 1mg Ask for price
Description: NADP disodium salt

NADP disodium salt

T22446-50mg 50mg Ask for price
Description: NADP disodium salt

NADP disodium salt

T22446-5mg 5mg Ask for price
Description: NADP disodium salt

DNQX disodium salt

T8459-10mg 10mg Ask for price
Description: DNQX disodium salt

DNQX disodium salt

T8459-1g 1g Ask for price
Description: DNQX disodium salt

DNQX disodium salt

T8459-1mg 1mg Ask for price
Description: DNQX disodium salt

DNQX disodium salt

T8459-50mg 50mg Ask for price
Description: DNQX disodium salt

DNQX disodium salt

T8459-5mg 5mg Ask for price
Description: DNQX disodium salt

NADH disodium salt

T5283-10mg 10mg Ask for price
Description: NADH disodium salt

NADH disodium salt

T5283-1g 1g Ask for price
Description: NADH disodium salt

NADH disodium salt

T5283-1mg 1mg Ask for price
Description: NADH disodium salt

NADH disodium salt

T5283-50mg 50mg Ask for price
Description: NADH disodium salt

NADH disodium salt

T5283-5mg 5mg Ask for price
Description: NADH disodium salt

NADH (disodium salt)

HY-F0001 10mM/1mL
EUR 135.6

NADP (disodium salt)

HY-F0002A 500mg
EUR 325.2

NADH disodium salt

MB270-100MG 1 unit
EUR 6.93
Description: NADH disodium salt

NADH disodium salt

MB270-1G 1 unit
EUR 35.37
Description: NADH disodium salt

NADH disodium salt

MB270-5G 1 unit
EUR 158.8
Description: NADH disodium salt

cGAMP disodium salt

2435-100 each
EUR 235.2

PIPES disodium salt

GB1799-100 100
EUR 79.1

PIPES disodium salt

GB1799-100G 100 g
EUR 132

PIPES disodium salt

GB1799-25 25
EUR 33.1

PIPES disodium salt

GB1799-250 250
EUR 150.4

PIPES disodium salt

GB1799-250G 250 g
EUR 217.2

PIPES disodium salt

GB1799-25G 25 g
EUR 84

PIPES disodium salt

GB1799-500 500
EUR 276.9

PIPES disodium salt

GB1799-500G 500 g
EUR 370.8

POPSO disodium salt

GB6170-100 100
EUR 237.2

POPSO disodium salt

GB6170-100G 100 g
EUR 322.8

POPSO disodium salt

GB6170-25 25
EUR 95

POPSO disodium salt

GB6170-25G 25 g
EUR 151.2

cGAMP disodium salt

GL2101-100 100
EUR 139.6

cGAMP disodium salt

GL2101-100UG 100 ug
EUR 192

cGAMP disodium salt

GL2101-500 500
EUR 340.2

cGAMP disodium salt

GL2101-500UG 500 ug
EUR 398.4

POPSO, disodium salt

PB4959 25g
EUR 92.36

Cromolyn Disodium Salt

C815000 1g
EUR 218
Description: 15826-37-6

Ticillin Disodium Salt

T437905 10g
EUR 127
Description: 4697-14-7

Ribarate Disodium Salt

R414200 250mg
EUR 1200
Description: 13035-93-3

Cefotetan disodium salt

GA5476-1 1
EUR 150.4

Cefotetan disodium salt

GA5476-100 100
EUR 55.4

Cefotetan disodium salt

GA5476-100MG 100 mg
EUR 103.2

Cefotetan disodium salt

GA5476-1G 1 g
EUR 217.2

Cefonicid Disodium Salt

C242875 10mg
EUR 305
Description: 190181-58-9

Carumonam Disodium Salt

C184745 25mg
EUR 49000
Description: 86832-68-0

Cefonicid disodium salt

574458 10.0mg
EUR 265

Sulisatin disodium salt

T34748-10mg 10mg Ask for price
Description: Sulisatin disodium salt

Sulisatin disodium salt

T34748-1g 1g Ask for price
Description: Sulisatin disodium salt

Sulisatin disodium salt

T34748-1mg 1mg Ask for price
Description: Sulisatin disodium salt

Sulisatin disodium salt

T34748-50mg 50mg Ask for price
Description: Sulisatin disodium salt

Sulisatin disodium salt

T34748-5mg 5mg Ask for price
Description: Sulisatin disodium salt

Etidronate disodium salt

GP3560-1 1
EUR 142.4

Etidronate disodium salt

GP3560-1G 1 g
EUR 208.8

Lobenzarit disodium salt

GP3753-100 100
EUR 197.7

Lobenzarit disodium salt

GP3753-100MG 100 mg
EUR 274.8

Fosfomycin Disodium Salt

F727500 100mg
EUR 92
Description: 26016-99-9

Methoxatin (disodium salt)

HY-100196A 10mM/1mL
EUR 223.2

ACT 373898 Disodium Salt

A191600 10mg
EUR 242

CL 316243 disodium salt

B6766-1 1 mg
EUR 62
Description: murine-selective β3 adrenoceptor agonist

CL 316243 disodium salt

B6766-10 10 mg
EUR 249
Description: murine-selective β3 adrenoceptor agonist

CL 316243 disodium salt

B6766-5 5 mg
EUR 162
Description: murine-selective β3 adrenoceptor agonist

CL 316243 Disodium Salt

C555000 25mg
EUR 19000
Description: 138908-40-4

LY 341495 disodium salt

T38169-10mg 10mg Ask for price
Description: LY 341495 disodium salt

LY 341495 disodium salt

T38169-1g 1g Ask for price
Description: LY 341495 disodium salt

LY 341495 disodium salt

T38169-1mg 1mg Ask for price
Description: LY 341495 disodium salt

Collectively, our findings indicate that MCP-1 related CHS conditioning increases immunomodulatory effects of MSC and enhanced their therapeutic efficacy in CHS. Improving the effectiveness of immunosuppressive MSC by preconditioning inflammatory cytokines associated with a particular disease may provide new strategies for MSC-based therapy for inflammatory disease.