Neuroinflammation has been involved in neurodegenerative diseases and acute brain injury such as stroke. monocytes chemotactic protein -1-induced protein -1 (Mcpip1) is a multifunctional protein action is known to have pro-apoptotic or anti-apoptotic depending on the nature of the experimental arrangement. However, its role in brain damage after asphyxia in the developing brain has not been studied. Therefore, we explored the role Mcpip1 on brain injury after hypoxia-ischemia in neonatal rats.

At postnatal day 7, Mcpip1-deficient and wild-type mice carotid artery ligation and exposure to hypoxia (8% oxygen). After a hypoxic-ischemic insult, we determine the time-course of cell death of apoptosis and expression levels of genes that encode proinflammatory factors. Mcpip1 impact on long-term brain damage rated one week after hypoxia-ischemia by cresyl violet staining.

We found caspase- 3 significantly increased activity in the ipsilateral brain tissue within 12-24 hours after hypoxia-ischemia. There is a marked increase in levels of mRNA transcripts that encode Mcpip1, TNF, and CCL2 in the ipsilateral brain tissue 6-48 hours after hypoxia-ischemia. We found caspase- hypoxia-ischemia-induced 3 and the activity levels of proinflammatory genes are inactivated in Mcpip1-KO mice compared to wild-type mice.

Histological assessment revealed that hypoxia-ischemia-induced brain tissue loss was significantly attenuated in the hippocampus of Mcpip1-KO mice compared to wild-type mice (9.0 ± 5.6% vs 3 3 0.9 ± 11.0%, P <0.05). Our data show that Mcpip1 contributes to acute and delayed brain damage, in part, through the regulation of neuroinflammation after hypoxic-ischemic insult in the developing rat brain.

Monocyte chemotactic protein-1-induced protein 1 contributes to neuronal injury following hypoxic-ischemia in the neonatal mouse brain
Monocyte chemotactic protein-1-induced protein 1 contributes to neuronal injury following hypoxic-ischemia in the neonatal mouse brain

MCP-1 priming Enhanced Mesenchymal stromal cells Therapeutic Effect on Contact Hypersensitivity by Enabling COX2-PGE2 / STAT3 Pathway mesenchymal stromal

cells (MSC) has become a promising treatment for inflammation-related diseases, and the efficacy of their therapy mainly depends on the crosstalk between the MSC and inflammation. However, methods to improve the efficiency of immunosuppressive of MSC in different diseases still need to be developed. In this study, we investigated whether preconditioning MSC inflammatory cytokines associated with the disease can improve their immunosuppressive properties and increasing the success of therapy.

In a mouse model of contact hypersensitivity (CHS), an inflammatory profile screening revealed that among all the cytokines tested, monocytes chemotactic protein -1 (MCP- 1) exhibited the lowest level increased significantly in the local microenvironment. As expected, MSC asphalt with MCP-1 (P-MSCs) exhibited an enhanced ability to downregulate the secretion of proinflammatory cytokines, induce regulatory T cells, inhibiting T cell proliferation, and the polarization of M2-type macrophages.

In vivo experiments showed that P-MSCs alleviated ear swelling and local production of proinflammatory cytokines is more effective than MSC controls. Mechanically, MCP-1 can significantly activate signal transducer and activator of transcription 3 (STAT 3 ) signaling pathway and induces the expression of cyclooxygenase-2 (COX2) and prostaglandin E2 (PGE2) in the MSC. STAT 3 inhibitor reversed MCP-1-mediated increase their immunosuppressive capabilities.

(RS)-MCPG disodium salt
B7476-100 100mg
EUR 1125
Description: group I/group II metabotropic glutamate receptor antagonist

(RS)-MCPG disodium salt
B7476-25 25mg
EUR 348
Description: group I/group II metabotropic glutamate receptor antagonist

(RS)-MCPG disodium salt
B7476-5 5mg
EUR 114
Description: group I/group II metabotropic glutamate receptor antagonist

ATP disodium salt
B3304-5.1 10 mM (in 1mL H2O)
EUR 129.6
Description: ATP Disodium salt is a P2 purinoceptor agonist.

ATP disodium salt
B3304-50 50 mg
EUR 153.6
Description: ATP Disodium salt is a P2 purinoceptor agonist.

ATP disodium salt
B3304-S Evaluation Sample
EUR 97.2
Description: ATP Disodium salt is a P2 purinoceptor agonist.

BCA disodium salt
2810-100G each
EUR 1038

BCA disodium salt
2810-1G each
EUR 144

BCA disodium salt
2810-500G each
EUR 3126

BCA disodium salt
2810-5G each
EUR 261.6

UDP disodium salt
B7278-5.1 10 mM (in 1mL DMSO)
EUR 129.6

UDP disodium salt
B7278-50 50 mg
EUR 150

ADA disodium salt
GB0256-100G 100 g
EUR 132

ADA disodium salt
GB0256-500G 500 g
EUR 409.2

ATP (disodium salt)
HY-B0345A 10mM/1mL
EUR 135.6

ATP disodium salt
B3304-1000 1g
EUR 43
Description: P2 purinoceptor agonist

ATP disodium salt
B3304-5000 5g
EUR 86
Description: P2 purinoceptor agonist

AMP Disodium Salt
40190012-1 25 g
EUR 75.46

AMP Disodium Salt
40190012-2 100 g
EUR 233.11

CTP Disodium Salt
40390000-1 50 mg
EUR 34.58

CTP Disodium Salt
40390000-2 100 mg
EUR 57.14

GTP Disodium salt
40790012-1 50 mg
EUR 37.26

GTP Disodium salt
40790012-2 100 mg
EUR 71.74

ADA disodium salt
GB0256-100 100
EUR 79.1

ADA disodium salt
GB0256-500 500
EUR 308.5

DNQX disodium salt
B5288-100 100 mg
EUR 334.8

DNQX disodium salt
B5288-25 25 mg
EUR 153.6

DNQX disodium salt
B5288-50 50 mg
EUR 223.2

NADH, disodium salt
2735-1000 each
EUR 202.8

NADH, disodium salt
2735-500 each
EUR 170.4

NADH, disodium salt
2735-5000 each
EUR 652.8

NADP, disodium salt
2736-100 each
EUR 183.6

NADP, disodium salt
2736-1000 each
EUR 940.8

NADP, disodium salt
2736-500 each
EUR 548.4

NBQX disodium salt
2759-25 each
EUR 770.4

NBQX disodium salt
2759-5 each
EUR 248.4

BCIP disodium salt
BB1171 500mg
EUR 279.24

NBQX disodium salt
B6566-10 10 mg
EUR 266.4

NBQX disodium salt
B6566-25 25 mg
EUR 466.8

NBQX disodium salt
B6566-5 5 mg
EUR 166.8

NBQX disodium salt
B6566-50 50 mg
EUR 819.6

CNQX disodium salt
B6567-1 1 mg
EUR 160.8

CNQX disodium salt
B6567-10 10 mg
EUR 258

CNQX disodium salt
B6567-50 50 mg
EUR 895.2

NADH (disodium salt)
HY-F0001 10mM/1mL
EUR 135.6

NADP (disodium salt)
HY-F0002A 500mg
EUR 325.2

R788 disodium salt
GW0002-1 1
EUR 212.7

R788 disodium salt
GW0002-10 10
EUR 533.3

R788 disodium salt
GW0002-5 5
EUR 311.9

DNQX disodium salt
B5288-10 10mg
EUR 113
Description: Selective non-NMDA receptor antagonist

NBQX disodium salt
B6566-1 1mg
EUR 182
Description: A potent, highly selective and competitive AMPA receptor antagonist

dCTP Disodium Salt
40390013-1 50 mg
EUR 71.74

dCTP Disodium Salt
40390013-2 100 mg
EUR 139.46

NADH Disodium Salt
41490008-1 1 g
EUR 41.18

NADH Disodium Salt
41490008-2 5 g
EUR 160.14

NADH Disodium Salt
41490008-3 50 g
EUR 1371.71

NADH, Disodium Salt
A3402-1G 1G
EUR 46.2
Description: High Purity

NADH, Disodium Salt
A3402-500MG 500MG
EUR 28.6
Description: High Purity

NADH, Disodium Salt
A3402-5G 5G
EUR 184.8
Description: High Purity

NADP, Disodium Salt
A4213-100MG 100MG
EUR 35.2
Description: High Purity

NADP, Disodium Salt
A4213-1G 1G
EUR 138.6
Description: High Purity

NADP, Disodium Salt
A4213-250MG 250MG
EUR 46.2
Description: High Purity

cGAMP disodium salt
2435-100 each
EUR 235.2

POPSO, disodium salt
PB4959 25g
EUR 92.36

cGAMP disodium salt
GL2101-100UG 100 ug
EUR 192

cGAMP disodium salt
GL2101-500UG 500 ug
EUR 398.4

PIPES disodium salt
GB1799-100G 100 g
EUR 132

PIPES disodium salt
GB1799-250G 250 g
EUR 217.2

PIPES disodium salt
GB1799-25G 25 g
EUR 84

PIPES disodium salt
GB1799-500G 500 g
EUR 370.8

POPSO disodium salt
GB6170-100G 100 g
EUR 322.8

POPSO disodium salt
GB6170-25G 25 g
EUR 151.2

PIPES disodium salt
GB1799-100 100
EUR 79.1

PIPES disodium salt
GB1799-25 25
EUR 33.1

PIPES disodium salt
GB1799-250 250
EUR 150.4

PIPES disodium salt
GB1799-500 500
EUR 276.9

POPSO disodium salt
GB6170-100 100
EUR 237.2

POPSO disodium salt
GB6170-25 25
EUR 95

cGAMP disodium salt
GL2101-100 100
EUR 139.6

cGAMP disodium salt
GL2101-500 500
EUR 340.2

Cefotetan disodium salt
GA5476-100MG 100 mg
EUR 103.2

Cefotetan disodium salt
GA5476-1G 1 g
EUR 217.2

Cefotetan disodium salt
GA5476-1 1
EUR 150.4

Cefotetan disodium salt
GA5476-100 100
EUR 55.4

Etidronate disodium salt
GP3560-1G 1 g
EUR 208.8

Lobenzarit disodium salt
GP3753-100MG 100 mg
EUR 274.8

Methoxatin (disodium salt)
HY-100196A 10mM/1mL
EUR 223.2

Etidronate disodium salt
GP3560-1 1
EUR 142.4

Lobenzarit disodium salt
GP3753-100 100
EUR 197.7

CL 316243 disodium salt
B6766-1 1 mg
EUR 130.8
Description: EC50: (3.0±0.3) X 10-8 M for ?3 adrenoceptor [1].Benzodioxole-containing phenethanolamine CL-316,243 is a murine-selective ?3 adrenoceptor agonist which can correct obesity and elevated blood glucose in diabetic rodents.

CL 316243 disodium salt
B6766-10 10 mg
EUR 351.6
Description: EC50: (3.0±0.3) X 10-8 M for ?3 adrenoceptor [1].Benzodioxole-containing phenethanolamine CL-316,243 is a murine-selective ?3 adrenoceptor agonist which can correct obesity and elevated blood glucose in diabetic rodents.

CL 316243 disodium salt
B6766-5 5 mg
EUR 247.2
Description: EC50: (3.0±0.3) X 10-8 M for ?3 adrenoceptor [1].Benzodioxole-containing phenethanolamine CL-316,243 is a murine-selective ?3 adrenoceptor agonist which can correct obesity and elevated blood glucose in diabetic rodents.

Fluorescein, disodium salt
FB0203 100g
EUR 76.7

Ticarcillin disodium salt
GA1874-100MG 100 mg
EUR 60

Ticarcillin disodium salt
GA1874-1G 1 g
EUR 132

Ticarcillin disodium salt
GA1874-500MG 500 mg
EUR 93.6

Balsalazide disodium salt
GP7647-1G 1 g
EUR 132

Fluorescein Disodium Salt
40600048-1 500 g
EUR 154.1

Fluorescein Disodium Salt
40600048-2 1 kg
EUR 272.69

Ticarcillin disodium salt
42010008-1 1 g
EUR 51.77

Ticarcillin disodium salt
42010008-2 5 g
EUR 89.68

Ticarcillin disodium salt
42010008-3 10 g
EUR 146.33

Ticarcillin disodium salt
42010008-4 25 g
EUR 329.23

Balsalazide disodium salt
GP7647-1 1
EUR 79.1

Ticarcillin disodium salt
GA1874-1 1
EUR 79.1

Ticarcillin disodium salt
GA1874-100 100
EUR 19.8

Ticarcillin disodium salt
GA1874-500 500
EUR 47.4

Hypoxanthine Disodium Salt
B1185-1G each
EUR 130.8

Hypoxanthine Disodium Salt
B1185-5G each
EUR 288

Phosphomycin disodium salt
GA7173-1G 1 g
EUR 84

Phosphomycin disodium salt
GA7173-25G 25 g
EUR 346.8

Phosphomycin disodium salt
GA7173-5G 5 g
EUR 160.8

Phosphomycin disodium salt
GA7173-1 1
EUR 39.4

Phosphomycin disodium salt
GA7173-25 25
EUR 257

Phosphomycin disodium salt
GA7173-5 5
EUR 102.7

Carbenicillin, Disodium Salt
A2511-5.1 10 mM (in 1mL DMSO)
EUR 129.6
Description: Carbenicillin inhibits the cell-wall synthesis (peptidoglycan cross-linking) by inactivating transpeptidase on the inner surface of the bacterial cell membrane.Carbenicillin is a white to slightly yellow, hygroscopic powder soluble in water and in alcohol.

Carbenicillin, Disodium Salt
A2511-50 50 mg
EUR 157.2
Description: Carbenicillin inhibits the cell-wall synthesis (peptidoglycan cross-linking) by inactivating transpeptidase on the inner surface of the bacterial cell membrane.Carbenicillin is a white to slightly yellow, hygroscopic powder soluble in water and in alcohol.

Carbenicillin disodium salt
GA1299-10G 10 g
EUR 274.8

Carbenicillin disodium salt
GA1299-1G 1 g
EUR 103.2

Carbenicillin disodium salt
GA1299-250MG 250 mg
EUR 70.8

Carbenicillin disodium salt
GA1299-25G 25 g
EUR 409.2

Carbenicillin disodium salt
GA1299-5G 5 g
EUR 189.6

Carbenicillin, Disodium Salt
A2511-1000 1g
EUR 67
Description: Inhibits the cell-wall synthesis,antibiotic

Carbenicillin, Disodium Salt
A2511-5000 5g
EUR 243
Description: Inhibits the cell-wall synthesis,antibiotic

Carbenicillin Disodium Salt
40310000-1 1 g
EUR 25.61

Carbenicillin Disodium Salt
40310000-2 5 g
EUR 51.79

Carbenicillin Disodium Salt
40310000-3 10 g
EUR 81.44

Carbenicillin Disodium Salt
40310000-4 25 g
EUR 142.47

Carbenicillin Disodium Salt
40310000-5 100 g
EUR 467.78

Carbenicillin, Disodium Salt
A2311-1G 1G
EUR 71.5
Description: Ultra Pure

Carbenicillin, Disodium Salt
A2311-5G 5G
EUR 255.2
Description: Ultra Pure

Carbenicillin disodium salt
GA1299-1 1
EUR 55.4

Carbenicillin disodium salt
GA1299-10 10
EUR 197.7

Carbenicillin disodium salt
GA1299-25 25
EUR 308.5

Carbenicillin disodium salt
GA1299-250 250
EUR 28.4

Carbenicillin disodium salt
GA1299-5 5
EUR 126.5

Phosphoramidon Disodium Salt
B4790-25 25 mg
EUR 588
Description: Phosphoramidon Disodium Salt is a potent inhibitor of metalloproteinase [1]. Metalloproteinase is an enzyme whose catalytic mechanism involves a metal. Most metalloproteases require zinc and some require cobalt.

Phosphoramidon Disodium Salt
B4790-5 5 mg
EUR 226.8
Description: Phosphoramidon Disodium Salt is a potent inhibitor of metalloproteinase [1]. Metalloproteinase is an enzyme whose catalytic mechanism involves a metal. Most metalloproteases require zinc and some require cobalt.

Protoporphyrin. disodium salt
P160-100MG 100 mg
EUR 140.46
Description: C34H34N4O4.Na2

Protoporphyrin. disodium salt
P160-25MG 25 mg
EUR 40.32
Description: C34H34N4O4.Na2

Collectively, our findings indicate that MCP-1 related CHS conditioning increases immunomodulatory effects of MSC and enhanced their therapeutic efficacy in CHS. Improving the effectiveness of immunosuppressive MSC by preconditioning inflammatory cytokines associated with a particular disease may provide new strategies for MSC-based therapy for inflammatory disease.