Neuroinflammation has been involved in neurodegenerative diseases and acute brain injury such as stroke. monocytes chemotactic protein -1-induced protein -1 (Mcpip1) is a multifunctional protein action is known to have pro-apoptotic or anti-apoptotic depending on the nature of the experimental arrangement. However, its role in brain damage after asphyxia in the developing brain has not been studied. Therefore, we explored the role Mcpip1 on brain injury after hypoxia-ischemia in neonatal rats.
At postnatal day 7, Mcpip1-deficient and wild-type mice carotid artery ligation and exposure to hypoxia (8% oxygen). After a hypoxic-ischemic insult, we determine the time-course of cell death of apoptosis and expression levels of genes that encode proinflammatory factors. Mcpip1 impact on long-term brain damage rated one week after hypoxia-ischemia by cresyl violet staining.
We found caspase- 3 significantly increased activity in the ipsilateral brain tissue within 12-24 hours after hypoxia-ischemia. There is a marked increase in levels of mRNA transcripts that encode Mcpip1, TNF, and CCL2 in the ipsilateral brain tissue 6-48 hours after hypoxia-ischemia. We found caspase- hypoxia-ischemia-induced 3 and the activity levels of proinflammatory genes are inactivated in Mcpip1-KO mice compared to wild-type mice.
Histological assessment revealed that hypoxia-ischemia-induced brain tissue loss was significantly attenuated in the hippocampus of Mcpip1-KO mice compared to wild-type mice (9.0 ± 5.6% vs 3 3 0.9 ± 11.0%, P <0.05). Our data show that Mcpip1 contributes to acute and delayed brain damage, in part, through the regulation of neuroinflammation after hypoxic-ischemic insult in the developing rat brain.

MCP-1 priming Enhanced Mesenchymal stromal cells Therapeutic Effect on Contact Hypersensitivity by Enabling COX2-PGE2 / STAT3 Pathway mesenchymal stromal
cells (MSC) has become a promising treatment for inflammation-related diseases, and the efficacy of their therapy mainly depends on the crosstalk between the MSC and inflammation. However, methods to improve the efficiency of immunosuppressive of MSC in different diseases still need to be developed. In this study, we investigated whether preconditioning MSC inflammatory cytokines associated with the disease can improve their immunosuppressive properties and increasing the success of therapy.
In a mouse model of contact hypersensitivity (CHS), an inflammatory profile screening revealed that among all the cytokines tested, monocytes chemotactic protein -1 (MCP- 1) exhibited the lowest level increased significantly in the local microenvironment. As expected, MSC asphalt with MCP-1 (P-MSCs) exhibited an enhanced ability to downregulate the secretion of proinflammatory cytokines, induce regulatory T cells, inhibiting T cell proliferation, and the polarization of M2-type macrophages.
In vivo experiments showed that P-MSCs alleviated ear swelling and local production of proinflammatory cytokines is more effective than MSC controls. Mechanically, MCP-1 can significantly activate signal transducer and activator of transcription 3 (STAT 3 ) signaling pathway and induces the expression of cyclooxygenase-2 (COX2) and prostaglandin E2 (PGE2) in the MSC. STAT 3 inhibitor reversed MCP-1-mediated increase their immunosuppressive capabilities.
ATP disodium salt |
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B3304-5.1 | ApexBio | 10 mM (in 1mL H2O) | EUR 129.6 |
Description: ATP Disodium salt is a P2 purinoceptor agonist. |
ATP disodium salt |
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B3304-50 | ApexBio | 50 mg | EUR 153.6 |
Description: ATP Disodium salt is a P2 purinoceptor agonist. |
ATP disodium salt |
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B3304-S | ApexBio | Evaluation Sample | EUR 97.2 |
Description: ATP Disodium salt is a P2 purinoceptor agonist. |
BCA disodium salt |
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2810-100G | Biovision | each | EUR 1038 |
BCA disodium salt |
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2810-1G | Biovision | each | EUR 144 |
BCA disodium salt |
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2810-500G | Biovision | each | EUR 3126 |
BCA disodium salt |
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2810-5G | Biovision | each | EUR 261.6 |
UDP disodium salt |
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B7278-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 129.6 |
UDP disodium salt |
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B7278-50 | ApexBio | 50 mg | EUR 150 |
ADA disodium salt |
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GB0256-100G | Glentham Life Sciences | 100 g | EUR 132 |
ADA disodium salt |
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GB0256-500G | Glentham Life Sciences | 500 g | EUR 409.2 |
ATP (disodium salt) |
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HY-B0345A | MedChemExpress | 10mM/1mL | EUR 135.6 |
DNQX disodium salt |
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B5288-100 | ApexBio | 100 mg | EUR 334.8 |
DNQX disodium salt |
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B5288-25 | ApexBio | 25 mg | EUR 153.6 |
DNQX disodium salt |
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B5288-50 | ApexBio | 50 mg | EUR 223.2 |
NADH, disodium salt |
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2735-1000 | Biovision | each | EUR 202.8 |
NADH, disodium salt |
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2735-500 | Biovision | each | EUR 170.4 |
NADH, disodium salt |
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2735-5000 | Biovision | each | EUR 652.8 |
NADP, disodium salt |
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2736-100 | Biovision | each | EUR 183.6 |
NADP, disodium salt |
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2736-1000 | Biovision | each | EUR 940.8 |
NADP, disodium salt |
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2736-500 | Biovision | each | EUR 548.4 |
NBQX disodium salt |
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2759-25 | Biovision | each | EUR 770.4 |
NBQX disodium salt |
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2759-5 | Biovision | each | EUR 248.4 |
BCIP disodium salt |
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BB1171 | Bio Basic | 500mg | EUR 279.24 |
NBQX disodium salt |
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B6566-10 | ApexBio | 10 mg | EUR 266.4 |
NBQX disodium salt |
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B6566-25 | ApexBio | 25 mg | EUR 466.8 |
NBQX disodium salt |
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B6566-5 | ApexBio | 5 mg | EUR 166.8 |
NBQX disodium salt |
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B6566-50 | ApexBio | 50 mg | EUR 819.6 |
CNQX disodium salt |
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B6567-1 | ApexBio | 1 mg | EUR 160.8 |
CNQX disodium salt |
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B6567-10 | ApexBio | 10 mg | EUR 258 |
CNQX disodium salt |
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B6567-50 | ApexBio | 50 mg | EUR 895.2 |
NADH (disodium salt) |
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HY-F0001 | MedChemExpress | 10mM/1mL | EUR 135.6 |
NADP (disodium salt) |
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HY-F0002A | MedChemExpress | 500mg | EUR 325.2 |
cGAMP disodium salt |
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2435-100 | Biovision | each | EUR 235.2 |
POPSO, disodium salt |
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PB4959 | Bio Basic | 25g | EUR 92.36 |
cGAMP disodium salt |
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GL2101-100UG | Glentham Life Sciences | 100 ug | EUR 192 |
cGAMP disodium salt |
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GL2101-500UG | Glentham Life Sciences | 500 ug | EUR 398.4 |
PIPES disodium salt |
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GB1799-100G | Glentham Life Sciences | 100 g | EUR 132 |
PIPES disodium salt |
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GB1799-250G | Glentham Life Sciences | 250 g | EUR 217.2 |
PIPES disodium salt |
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GB1799-25G | Glentham Life Sciences | 25 g | EUR 84 |
PIPES disodium salt |
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GB1799-500G | Glentham Life Sciences | 500 g | EUR 370.8 |
POPSO disodium salt |
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GB6170-100G | Glentham Life Sciences | 100 g | EUR 322.8 |
POPSO disodium salt |
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GB6170-25G | Glentham Life Sciences | 25 g | EUR 151.2 |
CL 316243 disodium salt |
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B6766-1 | ApexBio | 1 mg | EUR 130.8 |
Description: EC50: (3.0±0.3) X 10-8 M for ?3 adrenoceptor [1].Benzodioxole-containing phenethanolamine CL-316,243 is a murine-selective ?3 adrenoceptor agonist which can correct obesity and elevated blood glucose in diabetic rodents. |
CL 316243 disodium salt |
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B6766-10 | ApexBio | 10 mg | EUR 351.6 |
Description: EC50: (3.0±0.3) X 10-8 M for ?3 adrenoceptor [1].Benzodioxole-containing phenethanolamine CL-316,243 is a murine-selective ?3 adrenoceptor agonist which can correct obesity and elevated blood glucose in diabetic rodents. |
CL 316243 disodium salt |
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B6766-5 | ApexBio | 5 mg | EUR 247.2 |
Description: EC50: (3.0±0.3) X 10-8 M for ?3 adrenoceptor [1].Benzodioxole-containing phenethanolamine CL-316,243 is a murine-selective ?3 adrenoceptor agonist which can correct obesity and elevated blood glucose in diabetic rodents. |
Cefotetan disodium salt |
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GA5476-100MG | Glentham Life Sciences | 100 mg | EUR 103.2 |
Cefotetan disodium salt |
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GA5476-1G | Glentham Life Sciences | 1 g | EUR 217.2 |
Etidronate disodium salt |
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GP3560-1G | Glentham Life Sciences | 1 g | EUR 208.8 |
Lobenzarit disodium salt |
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GP3753-100MG | Glentham Life Sciences | 100 mg | EUR 274.8 |
Methoxatin (disodium salt) |
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HY-100196A | MedChemExpress | 10mM/1mL | EUR 223.2 |
Fluorescein, disodium salt |
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FB0203 | Bio Basic | 100g | EUR 76.7 |
Ticarcillin disodium salt |
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GA1874-100MG | Glentham Life Sciences | 100 mg | EUR 60 |
Ticarcillin disodium salt |
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GA1874-1G | Glentham Life Sciences | 1 g | EUR 132 |
Ticarcillin disodium salt |
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GA1874-500MG | Glentham Life Sciences | 500 mg | EUR 93.6 |
Balsalazide disodium salt |
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GP7647-1G | Glentham Life Sciences | 1 g | EUR 132 |
Hypoxanthine Disodium Salt |
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B1185-1G | Biovision | each | EUR 130.8 |
Hypoxanthine Disodium Salt |
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B1185-5G | Biovision | each | EUR 288 |
Phosphomycin disodium salt |
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GA7173-1G | Glentham Life Sciences | 1 g | EUR 84 |
Phosphomycin disodium salt |
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GA7173-25G | Glentham Life Sciences | 25 g | EUR 346.8 |
Phosphomycin disodium salt |
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GA7173-5G | Glentham Life Sciences | 5 g | EUR 160.8 |
ADA buffer, disodium salt |
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AB0005 | Bio Basic | 25g | EUR 79.84 |
Carbenicillin, Disodium Salt |
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A2511-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 129.6 |
Description: Carbenicillin inhibits the cell-wall synthesis (peptidoglycan cross-linking) by inactivating transpeptidase on the inner surface of the bacterial cell membrane.Carbenicillin is a white to slightly yellow, hygroscopic powder soluble in water and in alcohol. |
Carbenicillin, Disodium Salt |
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A2511-50 | ApexBio | 50 mg | EUR 157.2 |
Description: Carbenicillin inhibits the cell-wall synthesis (peptidoglycan cross-linking) by inactivating transpeptidase on the inner surface of the bacterial cell membrane.Carbenicillin is a white to slightly yellow, hygroscopic powder soluble in water and in alcohol. |
Carbenicillin disodium salt |
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GA1299-10G | Glentham Life Sciences | 10 g | EUR 274.8 |
Carbenicillin disodium salt |
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GA1299-1G | Glentham Life Sciences | 1 g | EUR 103.2 |
Carbenicillin disodium salt |
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GA1299-250MG | Glentham Life Sciences | 250 mg | EUR 70.8 |
Carbenicillin disodium salt |
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GA1299-25G | Glentham Life Sciences | 25 g | EUR 409.2 |
Carbenicillin disodium salt |
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GA1299-5G | Glentham Life Sciences | 5 g | EUR 189.6 |
Eosin Y disodium salt (C.I. 45380) |
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GT2884-100G | Glentham Life Sciences | 100 g | EUR 93.6 |
Eosin Y disodium salt (C.I. 45380) |
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GT2884-250G | Glentham Life Sciences | 250 g | EUR 151.2 |
Eosin Y disodium salt (C.I. 45380) |
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GT2884-25G | Glentham Life Sciences | 25 g | EUR 55.2 |
Phosphoramidon Disodium Salt |
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B4790-25 | ApexBio | 25 mg | EUR 588 |
Description: Phosphoramidon Disodium Salt is a potent inhibitor of metalloproteinase [1]. Metalloproteinase is an enzyme whose catalytic mechanism involves a metal. Most metalloproteases require zinc and some require cobalt. |
Phosphoramidon Disodium Salt |
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B4790-5 | ApexBio | 5 mg | EUR 226.8 |
Description: Phosphoramidon Disodium Salt is a potent inhibitor of metalloproteinase [1]. Metalloproteinase is an enzyme whose catalytic mechanism involves a metal. Most metalloproteases require zinc and some require cobalt. |
Pamoic acid disodium salt |
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B7638-10 | ApexBio | 10 mg | EUR 576 |
Pamoic acid disodium salt |
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B7638-50 | ApexBio | 50 mg | EUR 2233.2 |
Pamoic acid disodium salt |
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GK6564-100G | Glentham Life Sciences | 100 g | EUR 103.2 |
Pamoic acid disodium salt |
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GK6564-250G | Glentham Life Sciences | 250 g | EUR 170.4 |
Pamoic acid disodium salt |
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GK6564-25G | Glentham Life Sciences | 25 g | EUR 64.8 |
MUP, disodium salt [4-Methylumbelliferyl phosphate, disodium salt] *CAS 22919-26-2* |
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11610 | AAT Bioquest | 25 mg | EUR 86 |
MUP, disodium salt [4-Methylumbelliferyl phosphate, disodium salt] *CAS 22919-26-2* |
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11612 | AAT Bioquest | 10 g | EUR 1099 |
Phosphocreatine disodium salt |
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B7648-25 | ApexBio | 25 mg | EUR 235.2 |
UDP-GlcNAc Disodium Salt |
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HY-112174 | MedChemExpress | 5mg | EUR 198 |
Dimethylglyoxime disodium salt |
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DD1221 | Bio Basic | 100g | EUR 76.7 |
EDTA, Disodium Salt, Dihydrate |
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CH032 | ABM | 500 g | EUR 195.6 |
EDTA, Disodium Salt, Dihydrate |
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CH033 | ABM | 1.0 kg | EUR 234 |
EDTA, Disodium Salt, Dihydrate |
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CH034 | ABM | 2.5 kg | EUR 400.8 |
MUP, disodium salt, trihydrate |
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M0740 | Bio Basic | 1g | EUR 112.2 |
EDTA disodium salt dihydrate |
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GE3023-100G | Glentham Life Sciences | 100 g | EUR 48 |
EDTA disodium salt dihydrate |
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GE3023-1KG | Glentham Life Sciences | 1 kg | EUR 84 |
EDTA disodium salt dihydrate |
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GE3023-250G | Glentham Life Sciences | 250 g | EUR 55.2 |
EDTA disodium salt dihydrate |
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GE3023-500G | Glentham Life Sciences | 500 g | EUR 64.8 |
EDTA disodium salt dihydrate |
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GE3023-5KG | Glentham Life Sciences | 5 kg | EUR 180 |
EDTA, disodium salt, dihydrate |
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EB0185 | Bio Basic | 500g | EUR 84.01 |
EDTA zinc (II), disodium salt |
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EB6666 | Bio Basic | 100g | EUR 77.75 |
EDTA Disodium Salt 0.02m (0.04n) |
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ED20021 | Scientific Laboratory Supplies | 1L | EUR 157.2 |
EDTA copper(II) disodium salt |
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EB0434 | Bio Basic | 250g | EUR 100.72 |
DL-Malic acid disodium salt |
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GX1468-100G | Glentham Life Sciences | 100 g | EUR 55.2 |
DL-Malic acid disodium salt |
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GX1468-500G | Glentham Life Sciences | 500 g | EUR 90 |
Clodronate disodium salt hydrate |
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GP6103-1G | Glentham Life Sciences | 1 g | EUR 103.2 |
5'-Guanylic acid (disodium salt) |
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HY-W010970 | MedChemExpress | 10mM/1mL | EUR 135.6 |
Boc-Cys(SO3H)-OH · disodium salt |
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A-3625.0001 | Bachem | 1.0g | EUR 138 |
Description: Sum Formula: C8H13NNa2O7S2; CAS# [163558-29-0] |
Boc-Cys(SO3H)-OH · disodium salt |
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A-3625.0005 | Bachem | 5.0g | EUR 370.8 |
Description: Sum Formula: C8H13NNa2O7S2; CAS# [163558-29-0] |
Boc-Cys(SO3H)-OH · disodium salt |
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A-3625.0025 | Bachem | 25.0g | EUR 1371.6 |
Description: Sum Formula: C8H13NNa2O7S2; CAS# [163558-29-0] |
5g Carbenicillin Disodium Salt P |
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46-100-RG | Scientific Laboratory Supplies | EACH | EUR 327.6 |
Fmoc-Cys(SO3H)-OH · disodium salt |
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B-2415.0001 | Bachem | 1.0g | EUR 138 |
Description: Sum Formula: C18H15NNa2O7S2; CAS# [163558-30-3] |
Fmoc-Cys(SO3H)-OH · disodium salt |
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B-2415.0005 | Bachem | 5.0g | EUR 370.8 |
Description: Sum Formula: C18H15NNa2O7S2; CAS# [163558-30-3] |
Fmoc-Cys(SO3H)-OH · disodium salt |
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B-2415.0025 | Bachem | 25.0g | EUR 1371.6 |
Description: Sum Formula: C18H15NNa2O7S2; CAS# [163558-30-3] |
L-Tyrosine disodium salt hydrate |
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GE4061-100G | Glentham Life Sciences | 100 g | EUR 100.8 |
L-Tyrosine disodium salt hydrate |
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GE4061-25G | Glentham Life Sciences | 25 g | EUR 57.6 |
L-Tyrosine disodium salt hydrate |
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GE4061-50G | Glentham Life Sciences | 50 g | EUR 73.2 |
Hydroxy naphthol blue disodium salt |
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GT4927-100G | Glentham Life Sciences | 100 g | EUR 122.4 |
Hydroxy naphthol blue disodium salt |
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GT4927-25G | Glentham Life Sciences | 25 g | EUR 74.4 |
Volumetric Sol EDTA (DiSodium Salt) 0.01785M (0.0357N) |
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ED2003571 | Scientific Laboratory Supplies | 1L | EUR 112.28 |
BCA (Bicinchoninic acid), disodium salt |
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BB0260 | Bio Basic | 5g | EUR 185.28 |
Uridine 5'-diphosphate disodium salt |
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GC9934-100MG | Glentham Life Sciences | 100 mg | EUR 88.8 |
Uridine 5'-diphosphate disodium salt |
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GC9934-1G | Glentham Life Sciences | 1 g | EUR 313.2 |
Uridine 5'-diphosphate disodium salt |
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GC9934-250MG | Glentham Life Sciences | 250 mg | EUR 132 |
Uridine 5'-diphosphate disodium salt |
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GC9934-25MG | Glentham Life Sciences | 25 mg | EUR 62.4 |
Uridine 5'-diphosphate disodium salt |
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GC9934-500MG | Glentham Life Sciences | 500 mg | EUR 194.4 |
EDTA magnesium disodium salt hydrate |
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GE3956-100G | Glentham Life Sciences | 100 g | EUR 79.2 |
EDTA magnesium disodium salt hydrate |
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GE3956-1KG | Glentham Life Sciences | 1 kg | EUR 294 |
EDTA magnesium disodium salt hydrate |
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GE3956-250G | Glentham Life Sciences | 250 g | EUR 122.4 |
EDTA magnesium disodium salt hydrate |
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GE3956-25G | Glentham Life Sciences | 25 g | EUR 54 |
EDTA magnesium disodium salt hydrate |
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GE3956-500G | Glentham Life Sciences | 500 g | EUR 184.8 |
EDTA manganese disodium salt hydrate |
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GX6009-100G | Glentham Life Sciences | 100 g | EUR 122.4 |
EDTA manganese disodium salt hydrate |
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GX6009-250G | Glentham Life Sciences | 250 g | EUR 208.8 |
EDTA manganese disodium salt hydrate |
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GX6009-25G | Glentham Life Sciences | 25 g | EUR 69.6 |
EDTA manganese disodium salt hydrate |
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GX6009-500G | Glentham Life Sciences | 500 g | EUR 322.8 |
L-?-Hydroxyglutaric Acid Disodium Salt |
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B2812-25 | Biovision | each | EUR 418.8 |
L-?-Hydroxyglutaric Acid Disodium Salt |
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B2812-5 | Biovision | each | EUR 157.2 |
DL-?-Hydroxyglutaric acid disodium salt |
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A3837-100 | ApexBio | 100 mg | EUR 223.2 |
Description: IC50: N/AD-?-Hydroxyglutaric acid, an ?-hydroxy acid formed from the hydrolysis of (R)-5-oxo-2-tetrahydrofurancarboxylic acid, is over-produced in the human neurometabolic disease D-?-hydroxyglutaric aciduria. |
DL-?-Hydroxyglutaric acid disodium salt |
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A3837-25 | ApexBio | 25 mg | EUR 141.6 |
Description: IC50: N/AD-?-Hydroxyglutaric acid, an ?-hydroxy acid formed from the hydrolysis of (R)-5-oxo-2-tetrahydrofurancarboxylic acid, is over-produced in the human neurometabolic disease D-?-hydroxyglutaric aciduria. |
DL-?-Hydroxyglutaric acid disodium salt |
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A3837-50 | ApexBio | 50 mg | EUR 184.8 |
Description: IC50: N/AD-?-Hydroxyglutaric acid, an ?-hydroxy acid formed from the hydrolysis of (R)-5-oxo-2-tetrahydrofurancarboxylic acid, is over-produced in the human neurometabolic disease D-?-hydroxyglutaric aciduria. |
NADH disodium salt, trihydrate, reduced |
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NB0642 | Bio Basic | 1g | EUR 98.63 |
Succinic acid disodium salt, anhydrous |
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GK7702-100G | Glentham Life Sciences | 100 g | EUR 51.6 |
Succinic acid disodium salt, anhydrous |
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GK7702-1KG | Glentham Life Sciences | 1 kg | EUR 112.8 |
Succinic acid disodium salt, anhydrous |
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GK7702-250G | Glentham Life Sciences | 250 g | EUR 62.4 |
Succinic acid disodium salt, anhydrous |
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GK7702-500G | Glentham Life Sciences | 500 g | EUR 79.2 |
Succinic acid disodium salt, anhydrous |
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GK7702-5KG | Glentham Life Sciences | 5 kg | EUR 294 |
Collectively, our findings indicate that MCP-1 related CHS conditioning increases immunomodulatory effects of MSC and enhanced their therapeutic efficacy in CHS. Improving the effectiveness of immunosuppressive MSC by preconditioning inflammatory cytokines associated with a particular disease may provide new strategies for MSC-based therapy for inflammatory disease.